Recombinant ITGA4 anticorps

N° du produit ABIN7543376

Cet anticorps Lapin Chimeric détecte spécifiquement ITGA4 dans FACS, IP, BR et InhA. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant ITGA4 anticorps (ABIN7543376)

Antigène: ITGA4 (Integrin alpha 4 (ITGA4))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp ITGA4 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: HP1-2

Détail du produit anti-ITGA4 anticorps

Fonction: Anti-Integrin alpha 4 [HP1/2], Rabbit IgG, kappa

Specificité: This antibody specifically binds epitope B of the alpha 4 chain of human integrin alpha 4 (CD49d). Integrins alpha-4/beta-1 (VLA-4) acts as a receptor for fibronectin and VCAM1. VLA-4 is atypical because it is mostly restricted to lymphoid and myeloid cells, and indirect evidence had suggested that it might be involved in various cell-cell interactions. In addition, VLA-4 has been shown to mediate T and B lymphocyte attachment to the heparin II binding fragment of human plasma fibronectin (FN). This antibody is also reported to cross react with integrin alpha-4/beta-7 (α4β7).

Réactivité croisée: Cobaye

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody can be used for the immunoprecipitation of VLA-4 from cell lysates (PMID: 2430809). This antibody is also capable of detecting integrin alpha 4 expressed on the surface of Ramos cells by flow cytometry (PMID: 2430809, 1689216). This antibody can also block adherence of CTT leukocytes to recombinant soluble VCAM 1-coated plates in vitro (PMID: 7686922). This antibody can also block the interaction of VLA-4 with fibronectin. This antibody does not cause cell aggregation (PMID: 1709929). This antibody can bind soluble integrin alpha-4 in an ELISA. This antibody also inhibited the binding of PAF-stimulated eosinophils to MAdCAM-1 transfectants. This antibody also inhibited binding of unstimulated eosinophils to soluble recombinant human (rh)VCAM-1 and fibronectin (Fn) (PMID: 8911148).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur ITGA4

Antigène: ITGA4 (Integrin alpha 4 (ITGA4))

Autre désignation: Integrin alpha 4

Sujet: CD49d, VLA-4, α4, VLA4, ITGA4, α4β1, α4 integrin, VLA4 heterodimer (α4β1), Integrin alpha-4, Integrin alpha-IV, VLA-4 subunit alpha, CD49 antigen-like family member D, B1 integrin very late activation antigen-4

UniProt: P13612

Pathways: Integrin Complex